https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 The Membrane Protein Sortilin Is a Potential Biomarker and Target for Glioblastoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52963 Wed 28 Feb 2024 16:09:43 AEDT ]]> ACTN4 regulates the stability of RIPK1 in melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37515 Wed 23 Aug 2023 09:36:21 AEST ]]> BRAF/MEK inhibitors promote CD47 expression that is reversible by ERK inhibition in melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30575 Wed 20 Mar 2019 12:05:15 AEDT ]]> Cooperativity of HOXA5 and STAT3 is critical for HDAC8 inhibition-mediated transcriptional Activation of PD-L1 in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32982 Wed 17 Nov 2021 16:28:27 AEDT ]]> Functional identification of a novel transcript variant of INPP4B in human colon and breast cancer cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31508 Wed 15 Dec 2021 16:08:41 AEDT ]]> Shikonin induces programmed death of fibroblast synovial cells in rheumatoid arthritis by inhibiting energy pathways https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49890 Wed 14 Jun 2023 17:16:17 AEST ]]> Tumor innervation is triggered by endoplasmic reticulum stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47072 Wed 13 Mar 2024 09:25:37 AEDT ]]> Noxa upregulation by oncogenic activation of MEK/ERK through CREB promotes autophagy in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19023 V600E or MEK downregulated Noxa, whereas activation of MEK/ERK caused its upregulation. In addition, introduction of BRAFV600E increased Noxa expression in melanocytes. Upregulation of Noxa was due to a transcriptional increase mediated by cAMP responsive element binding protein, activation of which was also increased by MEK/ERK signaling in melanoma cells. Significantly, Noxa appeared necessary for constitutive activation of autophagy, albeit at low levels, by MEK/ERK in melanoma cells. Furthermore, it was required for autophagy activation that delayed apoptosis in melanoma cells undergoing nutrient deprivation. These results reveal that oncogenic activation of MEK/ERK drives Noxa expression to promote autophagy, and suggest that Noxa has an indirect anti-apoptosis role in melanoma cells under nutrient starvation conditions.]]> Wed 11 Apr 2018 16:41:25 AEST ]]> MEK-independent survival of B-RAFV600E melanoma cells selected for resistance to apoptosis induced by the RAF inhibitor PLX4720 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15166 V600E melanoma cells to B-RAF inhibitors. Experimental Design: B-RAFV600E melanoma cells were exposed to the B-RAF inhibitor PLX4720 for prolonged periods to select for cells resistant to apoptosis induced by the inhibitor. The resultant cells were analyzed for activation of extracellular signal regulated kinase (ERK), MAP/ERK kinase (MEK), and Akt, and related signals. Their roles in survival of the cells were also examined. Results: B-RAFV600E melanoma cells selected for resistant to PLX4720-induced apoptosis retained the V600E mutation in B-RAF, and proliferated steadily in the presence of the inhibitor, albeit with slow growth rate. These cells displayed high levels of ERK activation, that is, at least in part, independent of the conventional RAF/MEK/ERK pathway, as MEK activation was low and inhibition of MEK did not significantly block activation of ERK. In contrast, extracellular signals appeared involved. This was associated with elevated activation of the phosphoinositide 3-kinase (PI3k)/Akt pathway and could be inhibited by serum starvation and inhibition of PI3k/Akt. Inhibition of MEK did not impact on survival of these cells, whereas serum starvation, inhibition of PI3K/Akt, and inhibition of ERK1/2 reduced their viability. Conclusions: These results indicate that sensitivity to induction of apoptosis may be a major determinant of long-term responses of B-RAFV600E melanomas to specific inhibitors and suggest that rebound melanoma growth after initial treatment with the inhibitors may not be responsive to MEK inhibitors, but may be susceptible to inhibition of the PI3k/Akt pathway.]]> Wed 11 Apr 2018 16:15:39 AEST ]]> Evidence for upregulation of Bim and the splicing factor SRp55 in melanoma cells from patients treated with selective BRAF inhibitors https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15167 Wed 11 Apr 2018 16:01:18 AEST ]]> Sortilin is associated with breast cancer aggressiveness and contributes to tumor cell adhesion and invasion https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27772 Wed 11 Apr 2018 15:54:06 AEST ]]> MicroRNA-149*, a p53-responsive microRNA, functions as an oncogenic regulator in human melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13811 Wed 11 Apr 2018 15:41:21 AEST ]]> 2-Deoxy-D-glucose enhances TRAIL-induced apoptosis in human melanoma cells through XBP-1-mediated up-regulation of TRAIL-R2 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6913 Wed 11 Apr 2018 15:38:21 AEST ]]> Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18030 Wed 11 Apr 2018 14:49:14 AEST ]]> OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14361 Wed 11 Apr 2018 13:27:51 AEST ]]> Regulation of endoplasmic reticulum stress induced Apoptosis in human melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:2753 Wed 11 Apr 2018 13:06:05 AEST ]]> RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28314 Wed 11 Apr 2018 12:40:55 AEST ]]> miR-10b promotes migration and invasion in nasopharyngeal carcinoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14359 -ΔΔCt method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.]]> Wed 11 Apr 2018 12:17:27 AEST ]]> PI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14353 Wed 11 Apr 2018 11:50:58 AEST ]]> Up-regulation of Mcl-1 is critical for survival of human melanoma cells upon endoplasmic reticulum stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5046 Wed 11 Apr 2018 10:25:08 AEST ]]> Suppression of endoplasmic reticulum stress-induced invasion and migration of breast cancer cells through the downregulation of heparanase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14383 Wed 11 Apr 2018 09:49:12 AEST ]]> Reactive oxygen species dictate the apoptotic response of melanoma cells to TH588 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28216 Wed 11 Apr 2018 09:38:40 AEST ]]> INPP4B is upregulated and functions as an oncogenic driver through SGK3 in a subset of melanomas https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22865 Wed 11 Apr 2018 09:31:09 AEST ]]> BAG3-dependent expression of Mcl-1 confers resistance of mutant KRAS colon cancer cells to the HSP90 inhibitor AUY922 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32985 Wed 10 Nov 2021 15:04:52 AEDT ]]> Neurotrophin receptors TrkA, p75NTR, and sortilin are increased and targetable in thyroid cancer https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36867 NTR, and the proneurotrophin receptor sortilin were analyzed with immunohistochemistry in a cohort of thyroid cancers (n = 128) and compared with adenomas and normal thyroid tissues (n = 62). TrkA was detected in 20% of thyroid cancers, compared with none of the benign samples (P = 0.0007). TrkA expression was independent of histologic subtypes but associated with lymph node metastasis (P = 0.0148), suggesting the involvement of TrkA in tumor invasiveness. Nerves in the tumor microenvironment were positive for TrkA. p75NTR was overexpressed in anaplastic thyroid cancers compared with papillary and follicular subtypes (P < 0.0001). Sortilin was overexpressed in thyroid cancers compared with benign thyroid tissues (P < 0.0001). Neurotrophin receptor expression was confirmed in a panel of thyroid cancer cell lines at the mRNA and protein levels. Functional investigations using the anaplastic thyroid cancer cell line CAL-62 found that siRNA against TrkA, p75NTR, and sortilin decreased cell survival and cell migration through decreased SRC and ERK activation. Together, these data reveal TrkA, p75NTR, and sortilin as potential therapeutic targets in thyroid cancer.]]> Wed 09 Feb 2022 15:52:42 AEDT ]]> Schwann cell stimulation of pancreatic cancer cells: a proteomic analysis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37505 Wed 02 Mar 2022 14:28:59 AEDT ]]> Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28215 Wed 02 Mar 2022 14:25:48 AEDT ]]> Tumor neurobiology and the war of nerves in cancer https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47842 Wed 01 Feb 2023 16:02:55 AEDT ]]> Proteome and secretome analysis of pancreatic cancer cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50314 Tue 18 Jul 2023 11:37:22 AEST ]]> Tumor innervation and clinical outcome in pancreatic cancer https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43873 Tue 04 Oct 2022 12:29:00 AEDT ]]> Time to Introduce Nerve Density in Cancer Histopathological Assessment https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51303 Thu 31 Aug 2023 14:21:09 AEST ]]> 3-Bromopyruvate enhances TRAIL-induced apoptosis in human nasopharyngeal carcinoma cells through CHOP-dependent upregulation of TRAIL-R2 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31285 Thu 28 Oct 2021 12:36:57 AEDT ]]> The membrane protein sortilin can be targeted to inhibit pancreatic cancer cell invasion https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37858 P = 0.0014). Sortilin inhibition by siRNA and the pharmacologic inhibitor AF38469 strongly reduced the adhesion and invasion of pancreatic cancer cells without affecting cell survival and viability. Sortilin inhibition also decreased the phosphorylation of the focal adhesion kinase in Tyr925. Together, these data show that sortilin contributes to pancreatic cancer invasion and could eventually be targeted in therapy.]]> Thu 24 Mar 2022 11:34:45 AEDT ]]> The double life of RIPK1 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50344 Thu 20 Jul 2023 14:26:55 AEST ]]> BRAF/MEK inhibitors promote CD47 expression that is reversible by ERK inhibition in melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32504 Thu 14 Apr 2022 10:59:36 AEST ]]> Human melanoma cells under endoplasmic reticulum stress acquire resistance to microtubule-targeting drugs through XBP-1-mediated activation of Akt https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7006 Sat 24 Mar 2018 08:37:51 AEDT ]]> Human melanoma cells under endoplasmic reticulum stress are more susceptible to apoptosis induced by the BH3 mimetic obatoclax https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7007 Sat 24 Mar 2018 08:37:51 AEDT ]]> TRAIL-induced apoptosis of human melanoma cells involves activation of caspase-4 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9621 Sat 24 Mar 2018 08:35:27 AEDT ]]> Glucose-regulated protein 78 antagonizes cisplatin and adriamycin in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:6918 Sat 24 Mar 2018 08:34:50 AEDT ]]> Contrasting effects of Nutlin-3 on TRAIL- and Docetaxel-induced Apoptosis due to upregulation of TRAIL-R2 and Mcl-1 in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10447 Sat 24 Mar 2018 08:08:03 AEDT ]]> Sustained IRE1 and ATF6 signaling is important for survival of melanoma cells undergoing ER stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21386 Sat 24 Mar 2018 08:05:03 AEDT ]]> Down-regulation of RIP1 by 2-deoxy-D-glucose sensitizes breast cancer cells to TRAIL-induced apoptosis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17365 Sat 24 Mar 2018 08:01:31 AEDT ]]> Activation of Jun N-terminal kinase is a mediator of vincristine-induced apoptosis of melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5683 Sat 24 Mar 2018 07:47:32 AEDT ]]> Inhibition of endoplasmic reticulum stress-induced apoptosis of melanoma cells by the ARC protein https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5319 Sat 24 Mar 2018 07:45:57 AEDT ]]> Involvement of vacuolar H⁺₋ATPase in killing of human melanoma cells by the sphingosine kinase analogue FTY720 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26890 Sat 24 Mar 2018 07:41:39 AEDT ]]> RIP1 kinase is an oncogenic driver in melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26954 Sat 24 Mar 2018 07:27:01 AEDT ]]> The melanoma-associated antigen MAGE-D2 suppresses TRAIL receptor 2 and protects against TRAIL-induced apoptosis in human melanoma cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23422 Sat 24 Mar 2018 07:13:54 AEDT ]]> ProNGF Expression and Targeting in Glioblastoma Multiforme https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50637 Mon 31 Jul 2023 16:21:11 AEST ]]> Expression of NGF/proNGF and Their Receptors TrkA, p75<sup>NTR</sup> and Sortilin in Melanoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51398 Mon 04 Sep 2023 14:57:50 AEST ]]> Targeting XBP1 mRNA splicing sensitizes glioblastoma to chemotherapy https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51948 Fri 22 Sep 2023 17:06:07 AEST ]]> Cold Shock Domain Containing E1 (CSDE1) Protein is Overexpressed and Can be Targeted to Inhibit Invasiveness in Pancreatic Cancer Cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41096 Fri 22 Jul 2022 18:07:18 AEST ]]> Inhibition of HSP90 by AUY922 preferentially kills mutant KRAS colon cancer cells by activating Bim through ER stress https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28221 Fri 16 Oct 2020 16:09:46 AEDT ]]>